Using Fluorescent Post-Labeling to Probe the Subcellular Localization of DNA-Targeted Platinum Anticancer Agents.

Using Fluorescent Post-Labeling To Probe the Subcellular Localization of DNA-Targeted Platinum Anticancer Agents.

Angew Chem Int Ed Engl. 2013 Feb 20;
Ding S, Qiao X, Suryadi J, Marrs GS, Kucera GL, Bierbach U

Green around the cells: A post-labeling method was developed to image a DNA-targeted platinum drug in cancer cells by confocal fluorescence microscopy. This was done using ligation chemistry between an azide-functionalized platinum-acridine anticancer drug and an alkyne-modified dye, Alexa Fluor 488 (green star, see figure). The platinum-acridine agent was shown to accumulate in the nucleoli of cancer cells (NCI-H460). HubMed – drug


New views on cellular uptake and trafficking of manufactured nanoparticles.

J R Soc Interface. 2013; 10(82): 20120939
Treuel L, Jiang X, Nienhaus GU

Nanoparticles (NPs) are of similar size to typical cellular components and proteins, and can efficiently intrude living cells. A detailed understanding of the involved processes at the molecular level is important for developing NPs designed for selective uptake by specific cells, for example, for targeted drug delivery. In addition, this knowledge can greatly assist in the engineering of NPs that should not penetrate cells so as to avoid adverse health effects. In recent years, a wide variety of experiments have been performed to elucidate the mechanisms underlying cellular NP uptake. Here, we review some select recent studies, which are often based on fluorescence microscopy and sophisticated strategies for specific labelling of key cellular components. We address the role of the protein corona forming around NPs in biological environments, and describe recent work revealing active endocytosis mechanisms and pathways involved in their cellular uptake. Passive uptake is also discussed. The current state of knowledge is summarized, and we point to issues that still need to be addressed to further advance our understanding of cellular NP uptake. HubMed – drug


Pyrene-Tagged Dendritic Catalysts Noncovalently Grafted onto Magnetic Co/C Nanoparticles: An Efficient and Recyclable System for Drug Synthesis.

Angew Chem Int Ed Engl. 2013 Feb 20;
Keller M, Collière V, Reiser O, Caminade AM, Majoral JP, Ouali A

Hold on to your palladium! Phosphines have been grafted on magnetic Co/C nanoparticles through ?-? interactions. The resulting Pd complexes showed high activity for Suzuki couplings and the system involving a dendritic ligand was recyclable, allowing the preparation of the drug Felbinac over 12 consecutive runs with minimal Pd leaching. After extraction with CH(2) Cl(2) , Felbinac met the requirements of the pharmaceutical industry (<5 ppm Pd). HubMed – drug



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