Hyperdry Human Amniotic Membrane (HD-AM) Is Useful Material for Tissue Engineering: Physical, Morphological Properties and Safety as the New Biological Material.

Hyperdry human amniotic membrane (HD-AM) is useful material for tissue engineering: Physical, morphological properties and safety as the new biological material.

J Biomed Mater Res A. 2013 Apr 16;
Okabe M, Kitagawa K, Yoshida T, Suzuki T, Waki H, Koike C, Furuichi E, Katou K, Nomura Y, Uji Y, Hayashi A, Saito S, Nikaido T

Human amniotic membrane (AM) has been widely used as graft biomaterial for a variety of clinical applications. But, there have some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed Hyper dry AM (HD-AM) using far-infrared rays, depression of air and microwaves and then sterilized by ?-ray irradiation. To elucidate the benefit of HD-AM as biological materials, it was to compare to the histological and physical properties of HD-AM with a freeze-dried AM (FD-AM) as typical cryopreserved methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. HD-AM has been kept the morphological structure of epithelium and connective tissues. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. At 18 months after transplanted, single and multi layer of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the follow-up periods of 6 months after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium. HubMed – depression


The effectiveness of allied health care in patients with ataxia: a systematic review.

J Neurol. 2013 Apr 16;
Fonteyn EM, Keus SH, Verstappen CC, Schöls L, de Groot IJ, van de Warrenburg BP

Many patients with cerebellar ataxia have serious disabilities in daily life, while pharmacological treatment options are absent. Therefore, allied health care is considered to be important in the management of these patients. The goal of this review is to evaluate scientific evidence for allied health care in cerebellar ataxia, to identify effective treatment strategies, and to give recommendations for clinical practice and further research. A systematic search for clinical trials concerning allied health care in cerebellar ataxias was conducted using the electronic databases of PubMed, Medline, Embase, Cinahl and Pedro, and references lists of articles, in the time period from 1980 up to and including December 2011 in English and Dutch. We identified 14 trials, of which the four best studies were formally of moderate methodological quality. There was a wide variation in disease entities and interventions. The combined data indicate that physical therapy may lead to an improvement of ataxia symptoms and daily life functions in patients with degenerative cerebellar ataxia (level 2), and in other diseases causing cerebellar ataxia (level 3). When added to physical therapy, occupational therapy might improve global functional status, and occupational therapy alone may diminish symptoms of depression (level 3). There are insufficient data for speech and language therapy. Despite the widespread use of allied health care interventions in cerebellar ataxia, there is a lack of good quality studies that have evaluated such interventions. We found some support for the implementation of physical therapy and occupational therapy, but more research is needed to develop recommendations for clinical practice. HubMed – depression


Electrocardiographic Studies of Romidepsin Demonstrate Its Safety and Identify a Potential Role for the KATP channel.

Clin Cancer Res. 2013 Apr 15;
Noonan AM, Eisch RA, Liewehr DJ, Sissung TM, Venzon D, Flagg TP, Haigney MC, Steinberg SM, Figg WD, Piekarz RL, Bates SE

PURPOSE: Romidepsin is a histone deacetylase inhibitor (HDI) approved for the treatment of both cutaneous and peripheral T cell lymphoma (CTCL and PTCL). During development, a thorough assessment of cardiac toxicity was performed. EXPERIMENTAL DESIGN: A phase II single-agent non-randomized study of romidepsin was performed in patients with CTCL and PTCL who had progressed after at least one prior systemic therapy. RESULTS: Results for the first 42 patients enrolled on the NCI 1312 Phase II study of romidepsin in CTCL or PTCL demonstrated no cardiac toxicity based on serial electrocardiograms, troponins and MUGA scans/echocardiograms. The cardiac assessments reported herein confirm the safety of romidepsin among 131 enrolled patients, while supporting a role for electrolyte replacement. Heart rate increased an average 11 bpm following romidepsin infusion; there was no evidence of increased arrhythmia. Criteria for potassium/magnesium replacement were met prior to 55% of 1365 romidepsin doses; an association with hypoalbuminemia was confirmed. We propose a mechanism for ST segment flattening and depression, the most common electrocardiogram abnormalities observed: HDI-induced alteration of the activity or expression of KATP channels. In addition, examination of the variants of the active transporter of romidepsin, ABCB1, demonstrated a trend towards smaller heart rate changes in the peri-infusion period among wild type compared to variant diplotypes. CONCLUSIONS: We conclude that in the context of appropriate attention to electrolyte levels, the data support the cardiac safety of romidepsin. HubMed – depression


Exogenous Interleukin-6 Facilitated the Contraction of the Colon in a Depression Rat Model.

Dig Dis Sci. 2013 Apr 16;
Zhang L, Hu L, Chen M, Yu B

BACKGROUND: Gut dysmotility is closely associated with proinflammatory cytokines both in irritable bowel syndrome and inflammatory bowel disease. There is a dose-response relationship between depression and these inflammatory cytokines. AIMS: In the present study, we aimed to investigate the effect of Interleukin-6 (IL-6) on colon motility in a rat model of depression induced by chronic unpredictable mild stress (CUMS). METHODS: The contraction of the circular muscle strips of proximal colon was monitored by a polygraph. IL-6 and IL-6 receptor (IL-6R) mRNA was assayed by real-time quantitative PCR. Immunohistochemistry staining was used to locate the IL-6 and IL-6R in the rat colon. RESULTS: IL-6 and IL-6R were expressed in the mucosal layer, smooth muscle cells, and myenteric plexus of the colon. Exogenous IL-6 (20 ng/ml) increased the contraction of the circular muscle strip. Pretreatment of tetrodotoxin (blocker of voltage-dependent Na(+) channel on nerve fiber) blocks the excitatory effect of IL-6 on the contraction of the colon in non-stressed rats, but partially inhibited IL-6-induced excitatory effect on the muscle strips in CUMS-treated rats. CONCLUSIONS: These results suggest that IL-6-induced the contraction of the colonic strip by acting on the gut’s nervous system and acting directly on the smooth muscle in rats with depression. HubMed – depression



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