Treating Tuberculosis: Time to Introduce Fixed-Dose Drug Combinations.

Treating Tuberculosis: Time to Introduce Fixed-Dose Drug Combinations.

J Young Pharm. 2012 10; 4(4): 199-200
Manikandan S

HubMed – drug


The Role of Phenethyl Isothiocyanate on Bladder Cancer ADM Resistance Reversal and Its Molecular Mechanism.

Anat Rec (Hoboken). 2013 Mar 13;
Tang K, Lin Y, Li LM

Adramycin (ADM) resistance is an essential aspect of bladder cancer treatment failure and phenethyl isothiocyanate (PEITC) has been found to exhibit antitumor properties; however, the effect and potential mechanism of PEITC on bladder cancer ADM resistance reversal is not fully clear. The aim of this study was to explore the role of PEITC in bladder cancer cells ADM resistance reversal and the underlying molecular mechanisms. In this report, we identified the role of PEITC on ADM resistance reversal of human bladder carcinoma T24/ADM cells, including an increased drug sensitivity to ADM, cell apoptosis rates, intracellular accumulation of Rhodamine-123 (Rh-123), an increased expression of DNA topoisomerase II (Topo-II), and a decreased expression of multidrug resistance gene (MDR1), multidrug resistance-associated protein (MRP1), bcl-2 and glutathione s transferase ? (GST-?).We also found that there was a decreased expression of NF-?B, Survivin, Twist, and p-Akt, and an increased expression of PTEN and p-JNK after PEITC treatment for T24/ ADM cells. The results indicated that PEITC might be used as a potential therapeutic strategy to ADM resistance through blocking Akt and activating MAPK pathway in human bladder carcinoma. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc. HubMed – drug


Tracing antibacterial compounds from Acalypha australis Linn. by spectrum-effect relationships and semi-preparative high-performance liquid chromatography.

J Sep Sci. 2013 Mar 12;
Xiao S, Zhang LF, Zhang X, Li SM, Xue FQ

Several Acalypha australis Linn. species are used in traditional medicine in Southeast Asia. In this work, the ultra-performance liquid chromatography/mass spectrometry fingerprints and the antibacterial activities of Acalypha australis Linn. were investigated. An in-depth discussion on the reliability of identifying and obtaining potentially active compounds by spectrum-effect relationship and semi-preparative high performance liquid chromatography was conducted. The result shows that gallic acid and a compound with molecular weight of 634.1 in the fingerprints were the main antibacterial compounds. Compared to the crude extract of Acalypha australis Linn., both compounds increase the antibacterial efficacy 10 to 20 times. Compounds with molecular weights of 154.0, 292.0, and 485.1 in the fingerprints were the auxiliary antibacterial compounds. Through the entire isolation procedure, we obtained these antibacterial compounds with purities of 92.53%, 87.98%, 90.73%, 89.36%, and 88.14% respectively. This work provides a general model of the combination of ultra-performance liquid chromatography/mass spectrometry fingerprinting and antibacterial test to study the spectrum-effect relationships of Acalypha australis Linn. This model can be used to discover further the active compounds of this herb. HubMed – drug