Drug and Alcohol Rehabilitation: [Binding Mechanism of Traditional Chinese Medicine Active Component 5-Hydroxymethyl-Furfural and HSA or BSA].

[Binding mechanism of traditional Chinese medicine active component 5-hydroxymethyl-furfural and HSA or BSA].

Filed under: Drug and Alcohol Rehabilitation

Yao Xue Xue Bao. 2012 Mar; 47(3): 385-92
Guo M, He L, Lu XW

A combination of spectral experiment and molecular modeling techniques has been used to characterize the binding mechanism between an active component 5-hydroxymethyl-furfural (5-HMF) of traditional Chinese medicine and human serum albumin (HSA) or bovine serum albumin (BSA). The interaction mechanism of 5-HMF binding with HSA/BSA is analyzed. Although the drug can bind with HSA/BSA to form stable complexes, there are some differences in the bond strength. The values of binding distances (r) are different and low, which indicated the occurrence of energy transfer. The drug had conformational effect on HSA/BSA, which resulted in different changes of hydrophobic environment of the binding domain in HSA/BSA. The ‘phase diagram’ of fluorescence revealed that the changes on the conformational pattern of proteins have been affected by drug conformed to the “all-or-none” pattern. The interactions between drug and protein influenced by Co(II) were also discussed. Its effects acting on 5-HMF-HSA/BSA interactions are different. The computational modeling method was used to study the interaction between 5-HMF and HSA/BSA. The results of molecular model studies revealed that the binding modes for drug-serum albumin systems are mainly hydrophobic interactions and hydrogen bonding. These results are in accordance with spectral results. The research results have given a better theoretical reference for the study of pharmacological mechanism of 5-hydroxymethyl-furfural.
HubMed – drug


[Advances in the study of synergistic effect of anti-biofilm agents].

Filed under: Drug and Alcohol Rehabilitation

Yao Xue Xue Bao. 2012 Mar; 47(3): 339-45
Wang CZ, Cheng HJ

Biofilms are communities of surface-associated bacteria or fungi embedded in a self-produced extracellular polymeric matrix that are notoriously difficult to be eradicated and are sources of many recalcitrant infections. Treatment for biofilm infection with any individual drug is always less effective, while the combinations of different types of drugs are superior to monotherapy concerning the removing of biofilms. This paper focus on research progress in recent years for synergistic effect of drugs in combination against biofilms formed by Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans.
HubMed – drug


[Research progress of layer-by-layer self-assembly technique in drug delivery].

Filed under: Drug and Alcohol Rehabilitation

Yao Xue Xue Bao. 2012 Mar; 47(3): 332-8
Yu FF, Zou H, Zhong YQ

Now the layer-by-layer self-assembling (LbL) technique has become an attention-getting reparative methodology for ultrathin film formation. Many scientists in different academic areas including bioengieering, medical science, drug controlled release, optoelectronics dive into this technology. Among of them, carriers with structures which can be flexibly controlled are more useful since functional structure units can be assembled in layer-by-layer fashion, which is simplicity, chemical mildness, concealment, can achieve targeted drug delivery and so on. In this review, we have discussed the advantage, development, influential factors and applications of LbL. We have focused on reviewing the applications and perspective of nanoparticles, microgels and capsules were both fabricated via the LbL assembling at drug delivery.
HubMed – drug


[Current applications of high-throughput DNA sequencing technology in antibody drug research].

Filed under: Drug and Alcohol Rehabilitation

Yao Xue Xue Bao. 2012 Mar; 47(3): 322-31
Yu X, Liu QG, Wang MR

Since the publication of a high-throughput DNA sequencing technology based on PCR reaction was carried out in oil emulsions in 2005, high-throughput DNA sequencing platforms have been evolved to a robust technology in sequencing genomes and diverse DNA libraries. Antibody libraries with vast numbers of members currently serve as a foundation of discovering novel antibody drugs, and high-throughput DNA sequencing technology makes it possible to rapidly identify functional antibody variants with desired properties. Herein we present a review of current applications of high-throughput DNA sequencing technology in the analysis of antibody library diversity, sequencing of CDR3 regions, identification of potent antibodies based on sequence frequency, discovery of functional genes, and combination with various display technologies, so as to provide an alternative approach of discovery and development of antibody drugs.
HubMed – drug


Homeless court rehabilitates offenders

Filed under: Drug and Alcohol Rehabilitation

A comprehensive review of the court's success is to be done by August and will examine rehabilitation in terms of health, alcohol and drug usage, tenancy and financial stability. Yesterday the council's Social and Community Development Forum heard an …
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