BRMS1 Sensitizes Breast Cancer Cells to ATP-Induced Growth Suppression.

BRMS1 Sensitizes Breast Cancer Cells to ATP-Induced Growth Suppression.

Biores Open Access. 2013 Apr; 2(2): 77-83
Zhang Y, Chin-Quee K, Riddle RC, Li Z, Zhou Z, Donahue HJ

Purinergic signaling may represent an effective target in cancer therapy because the expression of purinergic receptors is altered in many forms of cancer and extracellular nucleotides modulate cancer cell growth. We examined the effect of extracellular ATP on the growth of the metastatic breast carcinoma cell line MDA-MB-435 relative to an immortalized breast epithelial cell line, hTERT-HME1. We also investigated whether the metastasis suppressor gene BRMS1 alters the sensitivity of breast cancer cells to ATP. Exposure to ATP for 24?h decreased proliferation and induced apoptosis in hTERT-HME1. However, exposure to ATP did not decrease proliferation or induce apoptosis in MDA-MD-435 cells until 48?h of exposure and only at higher doses than were effective with hTERT-HME1, suggesting MDA-MB-435 cells were resistant to the antiproliferative and apoptosis-inducing effects of ATP. Exposure to ATP for 24?h induced a decrease in proliferation of MDA-MB-435 cells expressing BRMS1, similar to hTERT-HME1, but did not induce an increase in apoptosis. MDA-MB-435 cells expressed low levels of the purinergic receptor P2Y2, as well as decreased ATP-induced cytosolic calcium mobilization, relative to hTERT-HME1. However, expressing BRMS1 in MDA-MB-435 cells restored P2Y2 levels and ATP-induced cytosolic calcium mobilization such that they were similar to hTERT-HME1. These data suggest that BRMS1 increases the sensitivity of breast cancer cells to the antiproliferative, but not apoptosis-inducing effects of ATP and that this is at least partly mediated by increased expression of the P2Y2 receptor. HubMed – rehab



Int J Sports Phys Ther. 2013 Apr; 8(2): 162-171
Lorenz DS, Beauchamp C

Lisfranc injuries are a challenging diagnosis for the sports physical therapist because of the lack of data on how to rehabilitate them properly. To date, the available rehabilitation literature has focused on the mechanism of injury and the conservative management of this injury. Furthermore, there is a lack of consensus on the appropriate testing and return to play criteria for an athlete recovering from this perplexing injury. This case describes a high school athlete whose primary sport was football, but was injured during wrestling. He suffered a Lisfranc injury and subsequently underwent surgical fixation. The purpose of this case report is to focus on the exercise, functional progression, and return to sport criteria utilized after operative treatment of a Lisfranc ligament injury.V. HubMed – rehab



Int J Sports Phys Ther. 2013 Apr; 8(2): 138-144
Negrete RJ, Hanney WJ, Pabian P, Kolber MJ

Agonist to antagonist strength data is commonly analyzed due to its association with injury and performance. The purpose of this study was to examine the agonist to antagonist ratio of upper body strength using two simple field tests (timed push up/timed modified pull up) in recreationally active adults and to establish the basis for reference standards.One hundred eighty (180) healthy recreationally active adults (111 females and 69 males, aged 18-45 years) performed two tests of upper body strength in random order: 1. Push-ups completed during 3 sets of 15 seconds with a 45 second rest period between each set and 2. Modified pull-ups completed during 3 sets of 15 seconds with a 45 second rest period between each set.The push-up to modified pull-up ratio for the males was 1.57:1, whereas females demonstrated a ratio of 2.72:1. The results suggest that for our group of healthy recreationally active subjects, the upper body “pushing” musculature is approximately 1.5-2.7 times stronger than the musculature involved for pulling.In this study, these recreationally active adults displayed greater strength during the timed push-ups than the modified pull-ups. The relationship of these imbalances to one’s performance and or injury risk requires further investigation. The reference values, however, may serve the basis for future comparison and prospective investigations. The field tests in this study can be easily implemented by clinicians and an agonist/antagonist ratio can be determined and compared to our findings.2b. HubMed – rehab



Int J Sports Phys Ther. 2013 Apr; 8(2): 121-128
Basnett CR, Hanish MJ, Wheeler TJ, Miriovsky DJ, Danielson EL, Barr JB, Grindstaff TL

Individuals with chronic ankle instability (CAI) often have impairments in ankle range of motion (ROM) and balance. There is limited evidence that these impairments are related in individuals with CAI. The purpose of this study was to determine the relationship between ankle dorsiflexion ROM and dynamic balance in individuals with CAI.Forty-five participants (age=23.2±2.8 y, height=172.1±10.8 cm, mass=70.6±13.3 kg, Foot and Ankle Ability Measure Sport= 71.2±11.7, Modified Ankle Instability Instrument= 6.4±1.3) volunteered for this study. Ankle dorsiflexion ROM was measured in a weight-bearing position while dynamic balance was measured using the Star Excursion Balance Test (SEBT) in the anterior, posteromedial, and posterolateral directions. Linear regression was used to determine the relationship between ankle dorsiflexion ROM and measures of dynamic balance.There were fair positive correlations between dorsiflexion ROM and the anterior reach direction (r = .55, r(2) = .31, P < .001), posterolateral reach direction (r = .29, r(2) = .09, P = .03), and the composite SEBT scores (r = .30, r(2) = .09, P= .02). There was little or no relationship between ankle dorsiflexion and the posteromedial reach direction (r = .01, r(2) = .001, P = .47).Ankle dorsiflexion ROM can influence dynamic balance, specifically the anterior reach portion of the SEBT.Individuals with CAI who demonstrate impairments in dorsiflexion ROM may also demonstrate difficulty with portions of the SEBT. Clinicians may use this information to better optimize rehabilitation programs that address ankle dorsiflexion ROM and dynamic balance.5. HubMed – rehab