Quantitative Tomographic Imaging of Intermolecular FRET in Small Animals.
Quantitative tomographic imaging of intermolecular FRET in small animals.
Filed under: Drug and Alcohol Rehabilitation
Biomed Opt Express. 2012 Dec 1; 3(12): 3161-75
Venugopal V, Chen J, Barroso M, Intes X
Forster resonance energy transfer (FRET) is a nonradiative transfer of energy between two fluorescent molecules (a donor and an acceptor) in nanometer range proximity. FRET imaging methods have been applied to proteomic studies and drug discovery applications based on intermolecular FRET efficiency measurements and stoichiometric measurements of FRET interaction as quantitative parameters of interest. Importantly, FRET provides information about biomolecular interactions at a molecular level, well beyond the diffraction limits of standard microscopy techniques. The application of FRET to small animal imaging will allow biomedical researchers to investigate physiological processes occurring at nanometer range in vivo as well as in situ. In this work a new method for the quantitative reconstruction of FRET measurements in small animals, incorporating a full-field tomographic acquisition system with a Monte Carlo based hierarchical reconstruction scheme, is described and validated in murine models. Our main objective is to estimate the relative concentration of two forms of donor species, i.e., a donor molecule involved in FRETing to an acceptor close by and a nonFRETing donor molecule.
HubMed – drug
Psoriatic Arthritis during Treatment with Bevacizumab for Anaplastic Oligodendroglioma.
Filed under: Drug and Alcohol Rehabilitation
Case Report Rheumatol. 2012; 2012: 208606
Graceffa D, Maiani E, Pace A, Solivetti FM, Elia F, De Mutiis C, Bonifati C
Bevacizumab is a recombinant humanised monoclonal antibody directed against the vascular endothelial growth factor (VEGF). The drug, alone or in combination with other anticancer agents, has been shown to be effective against several types of neoplasms. We report a case of a woman with a history of severe psoriasis who developed psoriatic arthritis during a course of bevacizumab, which was administered for a malignant glioma.
HubMed – drug
Development of Combination HIV Prevention Programs for People Who Inject Drugs through Government and Civil Society Collaboration in the Russian Federation.
Filed under: Drug and Alcohol Rehabilitation
Adv Prev Med. 2012; 2012: 874615
Volik MV, Karmanova GA, Berezina EB, Kresina TF, Sadykova RG, Khalabuda LN, Fattakhov FZ
Population Services International (PSI) has worked collaboratively with several government institutions of the Russian Federation to develop and implement a model program to access health services for individuals who are opioid dependent, including those with HIV infection. Through the development of partnership agreements between government organizations (GOs) and non-government organizations (NGOs), a model of the continuum of care has been developed that identifies a Recommended Package of HIV Prevention Services for Injecting Drug Users (RPS-IDU). The implementation of the RPS-IDU in the Russian Federation offers a model for other countries with HIV epidemics associated with injection drug use. This paper will describe the model program and its implementation in one of the pilot program regions.
HubMed – drug
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