(1)H NMR Detection of Small Molecules in Human Urine With a Deep Cavitand Synthetic Receptor.
(1)H NMR detection of small molecules in human urine with a deep cavitand synthetic receptor.
Filed under: Drug and Alcohol Rehabilitation
Analyst. 2013 Jan 10;
Ryan DA, Rebek J
A water-soluble deep cavitand recognized alkylammonium salts, including the drug amantadine hydrochloride, in spiked samples of human urine. The signals of the guests are detected by (1)H NMR upfield of 0 ppm and so occur in a spectroscopic window that is outside of the normal region and distinct from the signals of the biofluid components.
HubMed – drug
An integrated nano-scale approach to profile miRNAs in limited clinical samples.
Filed under: Drug and Alcohol Rehabilitation
Am J Clin Exp Immunol. 2012 Nov 30; 1(2): 70-89
Seumois G, Vijayanand P, Eisley CJ, Omran N, Kalinke L, North M, Ganesan AP, Simpson LJ, Hunkapiller N, Moltzahn F, Woodruff PG, Fahy JV, Erle DJ, Djukanovic R, Blelloch R, Ansel KM
Profiling miRNA expression in cells that directly contribute to human disease pathogenesis is likely to aid the discovery of novel drug targets and biomarkers. However, tissue heterogeneity and the limited amount of human diseased tissue available for research purposes present fundamental difficulties that often constrain the scope and potential of such studies. We established a flow cytometry-based method for isolating pure populations of pathogenic T cells from bronchial biopsy samples of asthma patients, and optimized a high-throughput nano-scale qRT-PCR method capable of accurately measuring 96 miRNAs in as little as 100 cells. Comparison of circulating and airway T cells from healthy and asthmatic subjects revealed asthma-associated and tissue-specific miRNA expression patterns. These results establish the feasibility and utility of investigating miRNA expression in small populations of cells involved in asthma pathogenesis, and set a precedent for application of our nano-scale approach in other human diseases. The microarray data from this study (Figure 7) has been submitted to the NCBI Gene Expression Omnibus (GEO; http://ncbi.nlm.nih.gov/geo) under accession no. GSE31030.
HubMed – drug
Incremental Healthcare Expenditures Associated with Thyroid Disorders among Individuals with Diabetes.
Filed under: Drug and Alcohol Rehabilitation
J Thyroid Res. 2012; 2012: 418345
Raval AD, Sambamoorthi U
Objective. To estimate incremental healthcare expenditures associated with thyroid disorders among individuals with diabetes. Research Design and Methods. Cross-sectional study design with data on adults over 20 years of age with diabetes (N = 4, 490) from two years (2007 and 2009) of the Medical Expenditure Panel Survey (MEPS) was used. Ordinary least square regressions on log-transformed total expenditures and type of healthcare expenditures (inpatient, emergency room, outpatient, prescription drug, and other) were performed to estimate the incremental expenditures associated with thyroid disorders after controlling for demographic, socioeconomic, health status, lifestyle risk factors, macrovascular comorbid conditions (MCCs), and chronic conditions (CCs). Results. Among individuals with diabetes, those with thyroid disorders had significantly greater average annual total healthcare expenditures ($ 15,182) than those without thyroid disorders ($ 11,093). Individuals with thyroid disorders had 34.3% greater total healthcare expenditures compared to those without thyroid disorders, after controlling for demographic, socio-economic, and perceived health status. Furthermore, controlling for CCs and MCCs, this increase in expenditures was reduced to 21.4%. Conclusions. Among individuals with diabetes, thyroid disorders were associated with greater healthcare expenditures; such excess expenditures may be due to CCs and MCCs. Comanagement of CCs and reducing MCCs may be a pathway to reduce high healthcare expenditures.
HubMed – drug
An alternative therapy for recurrent stasis ulcers in chronic venous insufficiency: venocuff.
Filed under: Drug and Alcohol Rehabilitation
Case Report Vasc Med. 2012; 2012: 315147
Yavuz C, Demirtas S, Guclu O, Karahan O, Yazici S, Caliskan A, Mavitas B
Chronic venous insufficiency may cause stasis ulcers that significantly impact on the quality of life. Many methods have been described for preventing or treating these ulcers. However, stasis ulcers often recur as a result of continuing venous insufficiency. Here we report a 30-year-old male patient with chronic venous insufficiency. He was admitted to the hospital owing to recurrent stasis ulcers. He had a history of various flavonoid drug usage and compression therapies over the previous six years. Venous Doppler sonography revealed combined saphenofemoral and deep femoral venous insufficiency. Venocuff was applied to the prejunctional and postjunctional parts of the femoral vein and the saphenofemoral junction. The patient was discharged on the postoperative second day, and a low-molecular-weight heparin dressing composed of calcium alginate was applied to the ulcer wound for one week after the operation. The stasis ulcer wound was totally healed after one month. The patient was followed up six months after the operation, and no postoperative complications or new ulceration was observed. Recurrent stasis ulcers are major reasons for hospitalization in patients with chronic venous insufficiency. Venocuff application for reducing venous insufficiency may be a good option for adjunctive ulcer therapy and for preventing recurrences of the problem.
HubMed – drug
Find More Drug And Alcohol Rehabilitation Information…