Identification of ACT-1 Plasmid-Mediated AmpC ?-Lactamase Producing Citrobacter Freundii From a Chinese Patient.

Identification of ACT-1 Plasmid-Mediated AmpC ?-Lactamase Producing Citrobacter freundii from a Chinese Patient.

Filed under: Rehab Centers

Ann Lab Med. 2013 Jan; 33(1): 86-8
Wei Y, Wang J

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First Detection of VIM-4 Metallo-?-Lactamase-Producing Citrobacter freundii in China.

Filed under: Rehab Centers

Ann Lab Med. 2013 Jan; 33(1): 84-5
Wei Y, Wang J

HubMed – rehab

 

Effects of sensory behavioral tasks on pain threshold and cortical excitability.

Filed under: Rehab Centers

PLoS One. 2013; 8(1): e52968
Volz MS, Suarez-Contreras V, Mendonca ME, Pinheiro FS, Merabet LB, Fregni F

Transcutaneous electrical stimulation has been proven to modulate nervous system activity, leading to changes in pain perception, via the peripheral sensory system, in a bottom up approach. We tested whether different sensory behavioral tasks induce significant effects in pain processing and whether these changes correlate with cortical plasticity.This randomized parallel designed experiment included forty healthy right-handed males. Three different somatosensory tasks, including learning tasks with and without visual feedback and simple somatosensory input, were tested on pressure pain threshold and motor cortex excitability using transcranial magnetic stimulation (TMS). Sensory tasks induced hand-specific pain modulation effects. They increased pain thresholds of the left hand (which was the target to the sensory tasks) and decreased them in the right hand. TMS showed that somatosensory input decreased cortical excitability, as indexed by reduced MEP amplitudes and increased SICI. Although somatosensory tasks similarly altered pain thresholds and cortical excitability, there was no significant correlation between these variables and only the visual feedback task showed significant somatosensory learning.Lack of correlation between cortical excitability and pain thresholds and lack of differential effects across tasks, but significant changes in pain thresholds suggest that analgesic effects of somatosensory tasks are not primarily associated with motor cortical neural mechanisms, thus, suggesting that subcortical neural circuits and/or spinal cord are involved with the observed effects. Identifying the neural mechanisms of somatosensory stimulation on pain may open novel possibilities for combining different targeted therapies for pain control.
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Identification of proteins from interstitium of trapezius muscle in women with chronic myalgia using microdialysis in combination with proteomics.

Filed under: Rehab Centers

PLoS One. 2012; 7(12): e52560
Olausson P, Gerdle B, Ghafouri N, Larsson B, Ghafouri B

Microdialysis (MD) of the trapezius muscle has been an attractive technique to investigating small molecules and metabolites in chronic musculoskeletal pain in human. Large biomolecules such as proteins also cross the dialysis membrane of the catheters. In this study we have applied in vivo MD in combination with two dimensional gel electrophoresis (2-DE) and mass spectrometry to identify proteins in the extracellular fluid of the trapezius muscle.Dialysate from women with chronic trapezius myalgia (TM; n?=?37), women with chronic wide spread pain (CWP; n?=?18) and healthy controls (CON; n?=?22) was collected from the trapezius muscle using a catheter with a cut-off point of 100 kDa. Proteins were separated by two-dimensional gel electrophoresis and visualized by silver staining. Detected proteins were identified by nano liquid chromatography in combination with tandem mass spectrometry.Ninety-seven protein spots were identified from the interstitial fluid of the trapezius muscle; 48 proteins in TM and 30 proteins in CWP had concentrations at least two-fold higher or lower than in CON. The identified proteins pertain to several functional classes, e.g., proteins involved in inflammatory responses. Several of the identified proteins are known to be involved in processes of pain such as: creatine kinase, nerve growth factor, carbonic anhydrase, myoglobin, fatty acid binding protein and actin aortic smooth muscle.In this study, by using in vivo microdialysis in combination with proteomics a large number of proteins in muscle interstitium have been identified. Several of the identified proteins were at least two-fold higher or lower in chronic pain patients. The applied techniques open up for the possibility of investigating protein changes associated with nociceptive processes of chronic myalgia.
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